HOT FIREPol® MultiPlex Mix is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers and water).
Generated PCR products are compatible with TA cloning procedures
Properties
Concentration: 5x
Hot-start: yes, initial activation in 12-15 min
Ready to load: no
Fidelity: 1 x Taq
Cloning Type: T/A cloning
Multiplexing: up to 18 targets (depending on amplicon length)
Amplicon Size: up to 5 kb
Mix Components
- HOT FIREPol® DNA polymerase
- 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution – 2 mM MgCl2)
- dNTPs
- BSA
- Compound that increases sample density (loading dyes not included)
