HOT FIREPol® MultiPlex Mix with 10 mM MgCl2

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  • analyze multiple targets per reaction
  • increased sensitivity and yield
  • prevents primer dimer formation
  • reaction set-up and shipment without dry ice
  • Special Requirements?

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    HOT FIREPol® MultiPlex Mix is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers and water).

    Generated PCR products are compatible with TA cloning procedures

    Properties

    Concentration: 5x

    Hot-start: yes, initial activation in 12-15 min

    Ready to load: no

    Fidelity: 1 x Taq

    Cloning Type: T/A cloning 

    Multiplexing: up to 18 targets (depending on amplicon length)

    Amplicon Size: up to 5 kb

    Mix Components

    • HOT FIREPol® DNA polymerase
    • 5x Multiplex Buffer with 10 mM MgCl2 (1x PCR solution – 2 mM MgCl2)
    • dNTPs 
    • BSA
    • Compound that increases sample density (loading dyes not included)
    Variant
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    Qty
    thumb_8306_18_HOT_FIREPol®_MultiPlex_Mix_10mM_MgCl2_1ml
    5 - ml (5-pack: 5 x 1 ml; 1250 rxn)
    5 ml (5-pack: 5 x 1 ml; 1250 rxn) / Pack

    SKU: SBD-04-34-00120-5

    Manufacturer Part No: 04-34-00120-5

    £440.63 inc. VAT
    thumb_8306_18_HOT_FIREPol®_MultiPlex_Mix_10mM_MgCl2_1ml
    10 - ml (10-pack: 10 x 1 ml; 2500 rxn)
    10 ml (10-pack: 10 x 1 ml; 2500 rxn) / Pack

    SKU: SBD-04-34-00120-10

    Manufacturer Part No: 04-34-00120-10

    £863.51 inc. VAT

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    HOT FIREPol® MultiPlex Mix with 10 mM MgCl2

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