µ-Plate 96 Well Black Glass Bottom

A black 96 well plate with square wells and a #1.5H glass coverslip bottom for high throughput screening using TIRF and super-resolution microscopy

• A microplate ideal for high-end cell microscopy due to black walls and a thin glass coverslip bottom
• Brilliant cell imaging thanks to the low thickness variability of the #1.5H glass coverslip and the excellent inner well and whole plate flatness
• Low well-to-well crosstalk in fluorescence microscopy

Applications

• Cultivation, high throughput screening (HTS), and high-resolution microscopy of cells
• Widefield fluorescence imaging and confocal microscopy of living and fixed cells
• Compound screenings (toxicology)
• Large-scale transfection experiments
• Live cell imaging, also suited for extended periods
• Total internal reflection fluorescence (TIRF) and single molecule applications
• Super-resolution microscopy (STED, SIM, (F)PALM, (d)STORM) and fluorescence correlation spectroscopy (FCS)
• Immunofluorescence staining

Technical Features

• Compatible with robotics and plate readers due to an ANSI/SLAS (SBS) standard format (85.5 x 127.5 mm)
• 96 quadratic wells with standard numbering (letters A–H and numbers 1–12)
• Suitable for fluorescence scanners
• Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
• May require coating to promote cell attachment
• No autofluorescence
• Excellent inner well flatness and whole plate flatness
• Compatible with staining and fixation solvents
• Sterile with single packaging
• Also available as µ-Plate 96 Well Black with a #1.5 ibidi Polymer Coverslip Bottom for optimized cell adhesion—for everyday use with diverse application possibilities

The Principle of the µ-Plate 96 Well Black Glass Bottom

The Coverslip Bottom

The µ-Plate 96 Well Black Glass Bottom comes with a high precision D263 M Schott glass, No 1.5H (170 +/– 5 µm) Glass Coverslip bottom. This bottom is ideally suitable for high-resolution microscopy and special microscopic applications like TIRF or super-resolution.