SoliSD™ Bsm DNA Polymerase Kit

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Exceptionally stable SoliSD™ Bsm DNA Polymerase for isothermal amplification

SoliSD™ Bsm DNA Polymerase Kit is provided in a flexible 5-vial format. Implemented Stability TAG and the unique SoliSD™ Supplement system improves the performance of the enzyme:

  • Stable for at least 1 month at room temperature
  • No NTC signal
  • Fast time to result from 4 minutes
  • Strong strand displacement activity
  • Active at 51-62°C with optimum at 60°C
  • Also available in glycerol-free format
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Description

SoliSD™ Bsm DNA Polymerase is based on the Large Fragment of the DNA Polymerase from the genus Bacillus smithii with functional similarity to other Bst and Bsm DNA polymerases. Implemented Stability TAG technology [1] ensures incredible stability for at least 1 month at 37°C.

SoliSD™ Bsm DNA Polymerase Kit is provided in a 5-vial format, where composition gives flexibility for developing your own isothermal amplification assay. The unique SoliSD™ Supplement system is provided with the kit. Technology improves the performance of the SoliSD™ Bsm DNA Polymerase:

  • Resolves common NTC signal issue
  • Minimizes variability between replicates
  • Allows reaction set-up at room temperature

The enzyme has a strong strand displacement DNA polymerase activity and lacks 5’→3’ and 3’→5’ exonuclease activities. It is designed for applications requiring synthesis through double-stranded DNA regions, such as loop-mediated isothermal amplification (LAMP).

[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016).

Reagents:

  • SoliSDTM Bsm DNA Polymerase (8 U/ µl)
  • 10x Isothermal Reaction Buffer (pH 8.8) 200 mM Tris-HCl, 150 mM (NH4)2SO4, 1% Tween-20, 250 mM KCl
  • 100 mM MgSO4 
  • 25x SoliSD Supplement
  • 10x GC-rich Enhancer
Item Pcs. Vial size
SoliSD™ Bsm DNA Polymerase (8 U/μl) 1 800 U | 100 μl
25x SoliSD™ Supplement 1 250 μl
10x Isothermal Reaction Buffer 1 1 ml
100 mM MgSO4 1 0.5 ml
10x GC-rich Enhancer 1 0.5 ml
Variant
Pack/Size
Ordering
Price
Qty
thumb_10192_SoliSD_kit_product_picture
250 rxn
250 / rxn

SKU: SBD-32-21-00250

Manufacturer Part No: 32-21-00250

UNSPSC Code: 41106314

£228.04 inc. VAT
thumb_10192_SoliSD_kit_product_picture
1000 rxn
1000 / rxn

SKU: SBD-32-21-01000

Manufacturer Part No: 32-21-01000

UNSPSC Code: 41106314

£866.22 inc. VAT
thumb_10192_SoliSD_kit_product_picture
5000 rxn
5000 / rxn

SKU: SBD-32-21-05000

Manufacturer Part No: 32-21-05000

UNSPSC Code: 41106314

£4,111.93 inc. VAT

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SoliSD™ Bsm DNA Polymerase Kit

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