Description
SOLIScript® Fast 1-step RT-qPCR Mix with UNG is optimized for probe-based one-step RT-qPCR assays. It contains all components necessary in a single vial (except water, template, primers and probes) to perform cDNA synthesis and qPCR with up to 5 targets within around 1h of total reaction time. Inhibitor tolerance and compatibility with fast cycling enable flexible experiment design. Salini UNG™ Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.
Applications
- Detection and quantification of RNA and DNA targets
- DNA/LNA hydrolysis probe-based assays
- Suitable for singleplex and multiplex reactions (up to 5 plex)
Properties
Sample type: RNA and DNA
Product format: Reagent kit (nuclease-free water supplied in a separate tube)
Detection type: Probe-based
Reference dye: none
Compatible real-time instruments: The product is compatible with most qPCR cyclers. If using a ROX-dependent platform, switch off ROX normalization prior to the run.
*Version with ROX available upon request.
Reagents
SOLIScript® Fast 1-step RT-qPCR Mix with UNG comprises:
- 4x SOLIScript® Fast 1-step RT-qPCR Mix with UNG (SOLIScript® Reverse Transcriptase, RiboGripTM RNase Inhibitor, HOT SolisFAST® DNA polymerase, Salini UNGTM Uracil-N-Glycosylase, a unique reaction buffer with dNTPs (including dUTPs), 22.0 mM MgCl2 (1x RT-qPCR solution – 5.5 mM MgCl2) and additives to maximize PCR efficiency).
- Water, nuclease-free
