RiboGrip®️ Glycerol-Free RNase Inhibitor (220 U/µl)

Description

RiboGrip^®️ Glycerol-Free RNase Inhibitor (220 U/µl) is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A, RNase B and RNase C.

RiboGrip^® inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. It is primarily used to prevent RNA degradation by contaminating RNases in various assays that use RNA sample materials, such as first-strand cDNA synthesis, RT-(q)PCR, RT-LAMP, etc.

RiboGrip^® also includes a genetic modification – Stability TAG – Solis BioDyne’s proprietary and patented polypeptide stabilization technology [1]. Stability TAG makes RiboGrip^® extremely tolerant to higher temperatures and enables room temperature shipping as well as effective use in assays requiring high incubation temperatures.

Highly concentrated 220 U/µl and glycerol-free formulation ensures compatibility with lyophilization and air-drying. This product does not contain excipients, please add your own as required or contact us for more information.

[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)

Applications

Compatible with lyophilisation and air-drying. Preventing RNA degradation in a variety of applications including:

• Assays involving lyophilisation or air-drying.
• First-strand cDNA synthesis
• RT-PCR, RT-qPCR, RT-LAMP
• In vitro transcription and translation
• RNA isolation and purification
• RNA sequencing

Properties

Concentration: 220 U/µl

Origin: Recombinant mammalian chimeric protein

Source: Purified from an E. coli strain that carries an overproducing plasmid containing a RiboGrip^® RNase Inhibitor gene.

Molecular weight: 47 kD monomer

Unit definition: One unit is defined as the amount of protein required to inhibit the activity of 5 ng of RNAse A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2′,3’‐cyclic monophosphate by RNase A.

Storage buffer: 25 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT and stabilizers.

Specificity: RNase A, RNase B, RNase C 

Recommendations for use: 0,5-2.5 U/μl final reaction concentration