PLA Cylinder with an Offset 6.35 Diameter Magnet to Create a Pellet Close to the Bottom of a 50 ml Tube for Low Volume Assays or Wash Steps, Mates to VP 772FB-1

Magnet insert for VP 772FB-1, PLA, low volume magnetic bead separation in 50ml conical tube, magnetic beads collect to side of conical portion of tube.

Magnetic Bead Separation Racks and Devices for Bottles, Tubes and Micro-tubes:

V&P has developed over 37 different magnetic bead separation racks and devices for bottles, tubes and micro-tubes, of nearly every description and covering the following range of rack size and vessel size.

# Single tube or bottle devices

# 4 tube or bottle racks, 6 tube rack, 8 tube rack, 10 tube rack, 12 tube rack, 14 tube rack, 16 tube rack, and a 24 tube rack,

# Bottle volumes of 100 ml, 500 ml, 1,000 ml, 2,000 ml and 5,000ml

# Tube volumes of 5 ml, 10 ml, 15 ml, 20 ml and 50 ml

# Micro-tube volumes of 0.2 ml, 0.5 ml, 1.5 ml and 2.0 ml

Match the Bottle, Tube or Micro-tube to the Magnetic Bead Separation Device:

It is important to match the right magnetic bead separation device with your particular bottle or tube. There are many characteristics of bottles and tubes that will affect where and how tightly the magnetic beads are pelleted:

# The thickness of the bottle or tube wall will determine how tightly the pellet is held.

# The smoothness of the interior surface will determine if beads will get trapped in crevices.

# The geometry of the vessel.

# The diameter of the vessel will determine how strong the magnets need to be.

# The shape and angle of the tube bottom will affect the angle of placement of the magnet.

# Your protocol will determine where the pellet should be located.

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All of the above features are very important, and we have dealt with all of them over the course of the last 10 years and that is why we say “choose your bottle or tube wisely before you launch a new assay protocol”. With our large selection of magnetic separation devices we can help you make the best decision for your application. We are happy to share our knowledge with you.

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Magnetic Beads:

We also consider the magnetic beads or particles you are using:

# Size of the beads

# Their magnetic moment

# Their concentration

We may be able to suggest a specific product for your application. If speed of separation is important to you, larger magnetic bead particles with greater magnetic moment will separate faster rather than weaker smaller particles.

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Magnetic Bead Separation Location:

Depending on your protocol, you may want the beads to be collected:

# On the side of the tubes

# High up the on the side of the tubes

# Low down on the side of tubes

# Or both high and low in the same Magnetic Separation device depending on different process, elution or wash volumes

# Directly in the bottom center of the tube

# On the bottom outside ring of the tube

# The mass or volume of the beads to be pelleted also determines which device is best

# The volume of supernatant and the method of removal (decanting, needle aspiration or pipetting) are also important considerations

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Magnetic Field Orientation and Strength:

The magnetic field orientation of adjacent magnets has a force multiplying effect (Halbach effect) and results in the magnetic beads being more tightly held as one side of the Halbach array has almost twice the strength and the opposite side has a very weak magnetic field:

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# We use Halbach arrays in several of our designs to improve pelleting

# The normal magnetic field strength of the magnet also affects how tightly the beads are held

# How close the magnet is placed to the magnetic beads also affects how tightly the beads are held

Types of supernatant separation – aspiration or pipet tip removal:

More commonly, magnetic beads are separated by aspiration or decanting from bottles. Magnetic beads pelleted in tubes are frequently removed by pipet and magnetic beads in micro-tubes are separated by pipet tips and then washed. When using pipets or pipet tips, it is important to keep the beads away from the pipet. This is why magnetic bead pellet location and how tightly it is magnetically held is so important.

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Advantages of V&P Scientific’s Magnetic Separation Devices

# Largest number of devices produced by any manufacturer

# Greatest variety of devices to fit all bottles, tube and micro-tubes

# Greatest variety for pellet location

# Strongest Magnets available in many different sizes

# Custom magnetic Separation Devices not a problem

# Our specialty is Flexibility

If you can’t find the rack for your procedure, call us and we will make a custom rack for you. It is not special, it just what we do to solve your problems.

Accessories

See our accessories for aspirating supernatants, adding wash solutions and for keeping magnetic beads uniformly in suspension prior to adding to tubes or microtubes. Recently we have developed a new SpinVessel^TM method that keeps magnetic beads in uniform suspension for aliquoting into tubes or microtubes.

Published Articles

Click here for a list of published articles that cite our Magnetic Separation Devices.

Applications for Magnetic Bead Separation Racks for micro-tubes, tubes and bottles.
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Since the introduction of Magnetic Beads as a method to separate bound from unbound ligands the applications and variation of Magnetic Bead Assays has soared exponentially, and they are literally too numerous to count. Magnetic Bead Separation Assays have rapidly replaced the slower and more cumbersome techniques of absorption (ELISA), centrifugation and filtration separation methods. In addition, Magnetic Bead assays have increased the recovery of sample and its purity with fewer wash steps.

Magnetic Beads have been successfully coupled to antibodies, antigens, proteins, viruses, bacteria, fungi, Ligands, DNA, RNA, Avidin, Streptavidin, Biotin, staphylococcal protein A and the list goes on…. Furthermore, these Magnetic beads have also been directly labeled with radio isotopes, enzymes, dyes, fluorescent compounds and complementary metal oxide semiconductors (CMOS) and quantum dots (QD) for direct detection with biosensors resulting in even more capability and multiplexing opportunities.

This said, Magnetic Bead Separation assays are the clear and obvious method of choice for the foreseeable future. V&P Scientific will work with developers of new Magnetic Bead assays to provide the perfect Magnetic Bead Separation device. We have already developed over 29 devices for micro-tubes, test tubes and bottles. Many of these are sold as a part of a kit for DNA polymerase binding and DNA cleanup on an OEM basis to diagnostic companies. Other applications include the capture and concentration of analytes from dilute solutions.

Applications of our Magnetic Bead Separation Blocks or Racks

1. DNA isolation for sequencing of genomes for identification of disease states
2. Diagnostic assays for disease-causing organisms.
3. DNA clean up prior to assay or PCR
4. Categorization of crops and animals in agriculture.
5. RNA extraction from cells for cancer detection.
6. RNA extraction from cells to analyze cell functions.
7. RNA extraction from cells for Gene Expression Profiling.
8. RNASeq assays used in biomicrofluidic lab-on-a-microchip technologies.
9. Purification and screening of antibodies, proteins, antigens and peptides.
10. Glycan analysis with Thermo Glycan Analysis mag bead kit
11. Isolate DNA from formalin-fixed, paraffin-embedded tissue.
12. Cell selection or sorting.
13. Retaining carryover magnetic beads when aspirating from deep well microplates.

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Compatible with the following microplate washers:

1. BioTek plate washer ELx405,
2. Tecan Hydrospeed plate washer,

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Compatible with the following magnetic beads:

1. Beckman Coulter AMPURE,
2. Beckman Coulter SPRI,
3. Luminex xMAP,
4. Promega,
5. Dynabeads,
6. MACHEREY-NAGEL,
7. MagSi-S,
8. PureProteome,

Other references describing magnetic bead separation blocks can be found at this URL.

We have also developed new devices for keeping magnetic beads in uniform suspension while being pipetted into microplates. See this link for dispensing uniform suspensions of magnetic beads using our new SpinVessel^TM system which has the advantage of operating with larger total volumes of magnetic beads and yet leaving a very tiny dead volume in the vessel. We have also recently developed a new SpinWash^TM system for washing Magnetic beads or concentrating dilute analytes.

See this link for our older sealed bearing magnetic bead suspension reservoirs and this link for Tumble Stirrer Magnetic bead suspension reservoirs.

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