HOT TERMIPol® DNA Polymerase (5 U/μl)

HOT TERMIPol^® DNA Polymerase

For in vitro use only

Description:

HOT TERMIPol^® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’→3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs).

HOT TERMIPol^® DNA polymerase is a chemically modified TERMIPol^® DNA polymerase. At ambient temperatures it is inactive, having no polymerase activity. HOT TERMIPol^® DNA polymerase is fully activated by a 8-12 minute incubation at 95°C. This prevents extension of non-specifically annealed primers.

Applications:

– Primer extension

– MALDI-TOF mass spectrometry

– Mass-array

Concentration:

5 U/µl

Reagents Provided:

– HOT TERMIPol^® DNA Polymerase

– 10x Reaction Buffer C 500 mM Tris-HCl pH 9.5 at 25°C

– 100 mM MgCl₂

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Shipping and Storage Conditions:

Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT TERMIPol^® DNA polymerase.