HOT TERMIPol® DNA Polymerase
For in vitro use only
Description:
HOT TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’→3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs).
HOT TERMIPol® DNA polymerase is a chemically modified TERMIPol® DNA polymerase. At ambient temperatures it is inactive, having no polymerase activity. HOT TERMIPol® DNA polymerase is fully activated by a 8-12 minute incubation at 95°C. This prevents extension of non-specifically annealed primers.
Applications:
- Primer extension
- MALDI-TOF mass spectrometry
- Mass-array
Concentration:
5 U/µl
Reagents Provided:
- HOT TERMIPol® DNA Polymerase
- 10x Reaction Buffer C 500 mM Tris-HCl pH 9.5 at 25°C
- 100 mM MgCl2
Storage and Dilution Buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Shipping and Storage Conditions:
Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT TERMIPol® DNA polymerase.
