HOT FIREPol® Probe qPCR Mix Plus (no ROX)

HOT FIREPol^® Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe.

The qPCR Mix contains optimized components and HOT FIREPol^® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol^® Probe qPCR Mix Plus (ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity.

HOT FIREPol^® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications

• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Microbial detection
• Viral load determination

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min

Detection type: Probe-based

Reference dye: ROX

Compatible real-time instruments: Cyclers that require ROX reference dye. Check Your cycler!

Mix Components

HOT FIREPol^® DNA Polymerase: chemically modified FIREPol^® DNA Polymerase enabling hot-start

5x Probe qPCR buffer with 15 mM MgCl₂: 1x PCR solution – 3 mM MgCl₂

dNTPs: dATP, dCTP, dGTP and dTTP

ROX reference dye