Solis BioDyne Oö

HOT FIREPol® Multiplex qPCR Mix

Probe-based qPCR master mix that has been optimized for highly sensitive and accurate quantification of up to 4 targets in a single reaction. This master mix was developed for TaqMan® probes but is also suitable for other hydrolysis probe types

  • analyze 1-4 targets in 1 reaction
  • high specificity and sensitivity
  • robust amplification of GC-rich targets
  • contains dUTP to prevent cross-contamination when
    used in combination with UNG
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    HOT FIREPol® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, and MgCl2.


    HOT FIREPol® Multiplex qPCR Mix is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity.


    HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

    Applications

    • detection and quantification of DNA and cDNA targets
    • profiling gene expression
    • microbial detection
    • viral load determination

    Properties

    Concentration: 5x

    Hot-start: yes, initial activation in 10 min

    Detection type: Probe-based

    Reference dye: none

    Suitable qPCR Cyclers: all cyclers that do not need passive reference dye. Check Your cycler!

    Mix Components

    HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start

    5x Multiplex qPCR buffer with 15 mM MgCl21x PCR solution – 3mM MgCl2

    dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs.
    IMPORTANT: UNG is not included in the HOT FIREPol® Multiplex qPCR Mix

    Variant
    Pack/Size
    Ordering
    Qty
    thumb_8319_89_HOT_FIREPol®_Multiplex_qPCR_Mix_ROX_1ml.jpg
    HOT FIREPol® Multiplex qPCR Mix - 10 - ml (10-pack: 10 x 1 ml; 2500 rxn)
    10 ml (10-pack: 10 x 1 ml; 2500 rxn) / Pack

    SKU: SBD-08-01-00001-10

    Manufacturer Part No: 08-01-00001-10

    thumb_8319_89_HOT_FIREPol®_Multiplex_qPCR_Mix_ROX_1ml.jpg
    HOT FIREPol® Multiplex qPCR Mix - 5 - ml (5-pack: 5 x 1 ml; 1250 rxn)
    5 ml (5-pack: 5 x 1 ml; 1250 rxn) / Pack

    SKU: SBD-08-01-00001-5

    Manufacturer Part No: 08-01-00001-5

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