HOT FIREPol® Multiplex qPCR Mix (ROX)

HOT FIREPol^® Multiplex qPCR Mix is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes, and template) to perform qPCR: HOT FIREPol^® DNA Polymerase, optimized buffer components, ultrapure dNTPs, and MgCl₂.

HOT FIREPol^® Multiplex qPCR Mix is optimized for DNA hydrolysis probes based on the 5′ flap endonuclease activity.

HOT FIREPol^® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications

• detection and quantification of DNA and cDNA targets
• profiling gene expression
• microbial detection
• viral load determination

Properties

Concentration: 5x

Hot-start: yes, initial activation in 10 min

Detection type: Probe-based

Reference dye: none

Suitable qPCR Cyclers: all cyclers that do not need passive reference dye. Check Your cycler!

Mix Components

HOT FIREPol^® DNA Polymerase: chemically modified FIREPol^® DNA Polymerase enabling hot-start

5x Multiplex qPCR buffer with 15 mM MgCl₂: 1x PCR solution – 3mM MgCl2

dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs.
IMPORTANT: UNG is not included in the HOT FIREPol^® Multiplex qPCR Mix