HOT FIREPol® GC Master Mix

HOT FIREPol^® GC Master Mix has been developed for working with difficult GC-rich templates and DNA secondary structures. It shows excellent amplification with templates up to 79% GC content

The master mix contains hot-start Taq polymerase HOT FIREPol^®, MgCl₂, dNTPs, and a special buffer for high yield GC-rich amplification.

Extra vials of 100% DMSO and 25 mM MgCl₂ enable flexibility in reaction optimization. 

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min.

Ready to load: no

Fidelity: 1 x Taq

Cloning Type: T/A cloning

Amplicon Size: up to 5 kb

Difficult templates: robust on GC-rich templates

Mix Composition:

HOT FIREPol^® DNA polymerase: chemically modified FIREPol^® DNA Polymerase enabling hot-start

5x HOT FIREPol^® GC Buffer with 7.5 mM MgCl₂: 1x PCR solution –1.5 mM MgCl₂

dNTPs: dATP, dCTP, dGTP and dTTP

Bovine serum albumin (BSA) to enhance PCR reaction

In separate vials:

100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.

25 mM MgCl₂ for further optimization