HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)

Dye-based real-time quantitative PCR (qPCR) uses DNA binding dye to evaluate the DNA amplification process during PCR. In this mix EvaGreen^® double-stranded DNA binding dye is used instead of the more widely used SYBR Green I that has similar fluorescence spectra. Compared to SYBR Green I dye EvaGreen^® dye shows a higher fluorescence level, high sensitivity for detecting low template concentrations, and high stability at room temperature. 

HOT FIREPol^® EvaGreen^® qPCR Mix Plus (ROX) is an optimized ready-to-use solution for dye-based real-time quantitative PCR assays on cyclers that require passive reference dye (including high ROX or low ROX reference signal requiring platforms).

Applications

• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Microbial detection
• Viral load determination

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min

Detection type: dye-based, includes EvaGreen^® intercalating dye

Reference dye: based on ROX

Compatible real-time instruments: Cyclers that require ROX reference dye. Check Your cycler!

Mix Components

HOT FIREPol^® DNA polymerase: chemically modified FIREPol^® DNA Polymerase enabling hot-start

5x EvaGreen^® qPCR buffer with 12. 5 mM MgCl₂: 1x PCR solution – 2.5 mM MgCl₂

dNTPs: dATP, dCTP, dGTP and dTTP

EvaGreen^® dye

Internal reference based on ROX dye

EvaGreen Dye

EvaGreen^® is a DNA-binding dye with many features that make it a superior alternative to SYBR^® Green I for qPCR. Apart from having similar spectra, EvaGreen^® has three important features that set it apart from SYBR^® Green I: EvaGreen^® has much less PCR inhibition, is an extremely stable dye, and has been shown to be non-mutagenic and non-cytotoxic. EvaGreen^® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR^® Green or FAM!