HOT FIREPol® DNA Polymerase (5 U/μl)

HOT FIREPol® DNA Polymerase

HOT START DNA Polymerase

Description:

HOT FIREPol^® DNA Polymerase is a chemically modified FIREPol^® DNA Polymerase. HOT FIREPol^® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extentension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

Applications:

– Hot Start PCR

– Primer extension

– TA cloning

Concentration:

5 U/µl

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents Provided:

– HOT FIREPol^® DNA Polymerase

– HOT FIREPol^® 10x Buffer B1 (Mg²⁺, detergent free) Tris-HCl and (NH₄)₂SO₄

– HOT FIREPol^® 10x Buffer B2 (Mg²⁺ free) Tris-HCl, (NH₄)₂SO₄ and detergent

– 25 mM MgCl₂

– 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution). Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

Shipping and Storage Conditions:

Routine storage: -20 °C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® DNA Polymerase.