HOT FIREPol® Blend Master Mix with 10 mM MgCl2

HOT FIREPol^® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers, and water).

HOT FIREPol^® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol^® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol^® Blend Master Mix exhibits an increased fidelity (up to fivefold) compared to regular Taq polymerase.

In addition, the mix also allows the amplification of longer fragments.

Generated PCR products are compatible with blunt-end and T/A cloning procedures.

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min.

Ready to load: no

Fidelity: 5 x Taq

Cloning Type: T/A cloning and Blunt-end cloning

Amplicon Size: up to 5 kb

Mix Composition:

HOT FIREPol^® DNA polymerase: chemically modified FIREPol^® DNA Polymerase enabling hot-start

Proofreading enzyme to enhance fidelity

5x Blend Master Mix Buffer with 7,5 mM MgCl₂: 1x PCR solution – 1,5 mM MgCl₂

1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

Bovine serum albumin (BSA) to enhance PCR reaction