HoloMonitor M4FL Fluorescence Unit

  • Combining holography together with fluorescence
  • Minimal light exposure to reduce photocytotoxicity
  • Small footprint that fits into your incubator
  • Compatible with HoloMonitor M4

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See your cells in a new light with holography and fluorescence imaging!

The HoloMonitor M4FL live cell imaging system comprises the compact, incubator-compatible HoloMonitor M4 live cell microscope and its add-on fluorescence module.

The combination of label-free holography and fluorescence imaging enables cell researchers to obtain unparalleled quantitative live cell data 24/7 while minimizing light exposure and reducing photocytotoxicity. The added fluorescence insights open up various new research applications.

Additional Key Applications with Fluorescence:

  • Cell Death
  • Co-Culture
  • Uptake Assay
  • Reporter Gene Expression
  • Transfection Efficiency
  • Live Cell Staining

HoloMonitor Applications – as seen in 270+ peer-reviewed publications worldwide:

  • Kinetic Cell Morphology (with data on 30+ parameters)
  • In-depth Single Cell Tracking & Analysis
  • Kinetic Cell Motility & Migration
  • Wound Healing (Scratch assay) | Chemotaxis
  • Kinetic Cell Proliferation
  • Cell Growth | Cell Division | Cell Cycle
  • Cell Differentiation
  • Kinetic Drug Dose-Response
  • Cytotoxicity | Cell Death
  • Cell Counter
  • Cell Quality Control (QC)

Why use HoloMonitor M4FL?

  • Work cell-friendly as it fits inside the incubator
  • Combine label-free holography with fluorescence
  • Decrease the number of fluorescence captures
  • Minimize photocytotoxicity
  • Get real-time quantitative data on single cells & cell populations
  • Study cell proliferation, morphology, and movement behavior at the same time
  • Save time with intuitive software & guided workflow developed by biologists
1-channel fluorescence Optimized for EGFP (FITC-Cy2)
Excitation 470 nm filtered LED, with bandwidth (FWHM): 40 nm
Emission 525 nm filtered, with bandwidth (FWHM): 50 nm
Exposure time 1 – 1000 ms
Field of view 0.25 mm²
Gain 1 – 4
Magnification 10x, matched with mounted HoloMonitor M4
Image Resolution 0.5 µm
Image size 1024 × 1024 pixels
Dimensions 310 x 180 x 85 mm (W × D × H)
Weight 4 kg
Power < 5 W
Interface USB connector

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