Fuse-It-mRNA for mRNA Transfection

A fusion reagent for rapid transfection of mRNA into the cytoplasm of living cells

• NEW: no sonication step required
• Immediate mRNA translation—protein synthesis detectable after 15–30 minutes
• Highly efficient and biocompatible, especially in primary cells (e.g., neurons or HUVECs) and stem cells

Applications

• mRNA translation and degradation studies
• Protein biochemistry studies: synthesis, folding, processing, stability, localization, degradation
• mRNA transfer into primary cells without generating genetically modified organisms (GMO)
• Genome engineering by RNA-only CRISPR/Cas technology

Technical Features

• Lipofection-independent transfer of mRNA into living cells
• mRNA transfer completed within 5–20 minutes
• No endocytosis involved
• No lysosomal degradation needed
• No gene transfer to the nucleus
• Protocol optimized for the transfer of functionally capped and polyadenylated mRNA
• No Biosafety Laboratory Level 1 or 2 necessary
• Excellent biocompatibility with low cytotoxicity

Specifications