FIREScript RT cDNA synthesis KIT
For in vitro use only
Description:
FIREScript RT cDNA Synthesis Kit contains all the reagents you need to synthesize cDNA in 7 separate vials. Its fast reaction speed allows you to perform your synthesis in just 15 minutes while the high working temperature of the enzyme (up to 60 °C) ensures high yield and great sensitivity. The kit format allows flexibility including the preincubation of complex RNA.
Applications:
- First strand cDNA synthesis
- RT-PCR
- RT-qPCR
Concentration:
200 U/µl
Storage and Dilution Buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 °C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents Provided:
- FIREScript Reverse Transcriptase
- RiboGrip RNase Inhibitor
- 10x RT Reaction Buffer with DTT
500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT
- Oligo (dT) Primer
- Random Primers
- dNTP Mix
- Water, nuclease free
Shipping and Storage Conditions:
Routine storage: -20 °C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
