FIREScript RT cDNA syntesis MIX

FIREScript RT cDNA Synthesis Mix

For in vitro use only

Description:

FIREScript RT cDNA Synthesis Mix contains all the reagents you need to synthesize cDNA from just 3 tubes. Its fast reaction speed allows you to perform your synthesis in just 15 minutes while the high working temperature of the enzyme (up to 60 °C) ensures high yield and great sensitivity.

Applications:

– First strand cDNA synthesis

– RT-PCR

– RT-qPCR

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25 °C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents Provided in Product Sample:

– FIREScript Enzyme Mix

FIREScript RT and RiboGrip RNase Inhibitor

– 10x RT Reaction Premix with Oligo (dT) and Random primers

RT Reaction Buffer with DTT, dNTPs, Oligo (dT) primer and Random primers

– 10x RT Reaction Premix with Oligo (dT) primer

RT Reaction Buffer with DTT, dNTPs and Oligo (dT) primer

– 10x RT Reaction Premix with Random primers

RT Reaction Buffer with DTT, dNTPs and Random primers

– 10x RT Reaction Premix without primers

RT Reaction Buffer with DTT and dNTPs

– Water, nuclease free

Reagents Provided:

– FIREScript Enzyme Mix

FIREScript RT and RiboGrip RNase Inhibitor

– 10x RT Reaction Premix

RT Reaction Buffer with DTT, dNTPs and priming method of choice*

– Water, nuclease free

*See product name for corresponding priming method.

Shipping and Storage Conditions:

Routine storage: -20 °C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.