Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.
Ready to Load format also includes a compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during electrophoresis.
We recommend using FIREPol® Master Mix Ready to Load in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining. FIREPol® Master Mix Ready to Load is not recommended for use in post-PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary because yellow and blue dyes can interfere with these applications.
Properties
Concentration: 5x
Hot-start: no
Ready to load: yes
Fidelity: 1x Taq
Cloning Type: T/A Cloning
Amplicon Sixe: up to 5 kb
Mix Components
FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
7.5 mM MgCl2: 1x PCR solution – 1.5 mM MgCl2
1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
Blue dye: migration equivalent to 3.5-4.5 kb DNA fragment
Yellow dye: migration equivalent to 35-45 bp DNA fragment
Compound that increases sample density for direct loading
