FIREPol® Master Mix (7.5 mM MgCl2)
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If you are looking for a quote, you can request this here.Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.
We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.
Properties
Concentration: 5x
Hot-start: no
Ready to load: no
Fidelity: same as Taq
Cloning Type: T/A Cloning
Amplicon Size: up to 5 kb
Mix Components
FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
Variant
Pack/Size
Price
Qty
5 - ml (5-pack: 5 x 1 ml; 1250 rxn)
5 ml (5-pack: 5 x 1 ml; 1250 rxn) / Pack
£180.34 inc. VAT
10 - ml (10-pack: 10 x 1 ml; 2500 rxn)
10 ml (10-pack: 10 x 1 ml; 2500 rxn) / Pack
£353.38 inc. VAT
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