FIREPol® Master Mix (12.5 mM MgCl2)

Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol^®, MgCl₂, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.

We recommend using FIREPol^® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.

Properties

Concentration: 5x

Hot-start: no

Ready to load: no

Fidelity: same as Taq

Cloning Type: T/A Cloning

Amplicon Size: up to 5 kb

Mix Components

FIREPol^® DNA polymerase: highly processive. thermostable DNA polymerase

5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH₄)₂SO₄, 0.1% w/v Tween-20

12.5 mM MgCl₂: 1x PCR solution – 2.5 mM MgCl₂

1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP