FIREPol® DNA Polymerase (5 U/μl)

FIREPol^® DNA Polymerase

For in vitro use only

Description:

FIREPol^® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol^® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

Applications:

– Suited for a wide range of PCR assays

– Primer extension

– TA cloning

Concentration:

5 U/µl

Storage and Dilution Buffer:

50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents Provided:

– FIREPol^® DNA Polymerase

– 10x Reaction buffer B (Mg²⁺ free)

0.8 M Tris-HCl, 0.2 M (NH₄)₂SO₄, 0.2% w/v Tween-20

– 10x Reaction buffer BD (Mg²⁺ and detergent free)

0.8 M Tris-HCl, 0.2 M (NH₄)₂SO₄

– 25 mM MgCl₂

– 10x Solution S Additive that facilitates amplification of difficult templates (e.g. GC-rich DNA templates). This solution should be used at a defined working concentration (1x, 2x or 3x solution). Solution S is NOT a reaction buffer and should be used ONLY IF non-specific amplifications occur.

Shipping and Storage Conditions:

Routine storage: -20 °C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol^® DNA Polymerase.