Athena Zebrafish Image Analysis Software

IDEA Bio-Medical is proud to present Athena Zebrafish – the next generation in Zebrafish assays. Our new, unique dedicated analysis software for automated analysis of Zebrafish microscopy images offers simple and quick quantification of fluorescence, measurement of morphological changes & other phenotypic features in Zebrafish larvae in a high throughput format.

Athena Zebrafish is suited for a broad range of researchers and accepts multiple image format types output from nearly all microscope manufacturers.

The software permits parameter-free zebrafish analysis using simple bright-field images. It automatically detects zebrafish embryos and larvae up to 5 days old (dpf), extracting the fish contour and much of its internal anatomy: yolk sac, eye, notocord, and more, along with body regions of the head, trunk, and tail.

For each of these objects, the software measures the morphology (area, length, and shape) and can detect fluorescence in associated color channels. Both fluorescence intensity and spot/structure detection within specific anatomy are supported.

Zebrafish (Danio rerio) are an attractive model organism for the study of human disease pathology because of their optical transparency and genetic tractability. They serve as a great alternative to mammalian screening due to cost, throughput and reduced ethical concerns.

Automated analysis of Zebrafish imposes unique demands due to the versatility of organs and features needed to be detected.

To increase throughput, robustness and permit unbiased image analysis of zebrafish, IDEA Bio-Medical developed a novel AI-based software to automatically  detect fish contour and internal anatomy in brightfield images.  By identifying these hard to detect structures, our software maintains the anatomical context of associated fluorescence signals to enable true high-content imaging in Zebrafish.  Thusly, zebrafish morphology along with region-specific spot/cell counting and fluorescence intensity measurement are readily quantified.

• Quick & simple user interface

• Designed for non-specialists in image analysis

• Compatible for images from commercial microscopes

• The fastest & easiest Zebrafish image-based screening tool available

• Novel Artificial Intelligence- based algorithms for fish & organ identification

• No user intervention required to readily detect fish & internal organs in brightfield

• Combine with fluorescent labeling for structural co-analysis

• Ensure proper fish orientation in post-analysis with customizable, software-based selection

• Software  suited for researchers who only image several fish per week, as well as researchers imaging hundreds & thousands of fish in multi-well plates for large scale screens.

• Software accepts any microscopy image, supporting multiple image formats from manual microscopes or automated imaging systems from vendors such as Leica, Zeiss, Nikon and more.

• Unbeatable throughput: analyze hundreds of Zebrafish larvae within minutes

• Analyzes  Label-free or fluorescently tagged fish and internal organelles

• Analyze your Fish larva from head to tail with supported image analysis from single plane, Z stack and projection images