5x SolisFAST™ SolisGreen® qPCR Mix (no ROX)

SolisFAST^® SolisGreen^® qPCR Mix (no ROX)

Fast and highly sensitive dye-based qPCR

For in vitro use only

Description:

SolisFAST® SolisGreen® qPCR Mix (no ROX) is a 5x-concentrated ready-to-use solution optimized for fast, highly sensitive and reproducible dye-based qPCR assays. Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start, optimized buffer and the SolisGreen® dye, this qPCR Mix offers robust qPCR, accurate target detection, ice free shipping and reaction set-up.

The mix contains all components necessary, except primers, nuclease-free water, and DNA template.

The mix is compatible with qPCR cyclers that do not need ROX as a passive reference signal for normalization of the data.

Applications:

– DNA/cDNA target detection

– Gene expression analysis

– Copy Number Variation (CNV) analysis

– miRNA profiling and quantification

Benefits:

• FAST – delivers reproducible qPCR results up to 2x faster
• ACCURATE – reliable quantification across technical replicates
• SENSITIVE – consistent results with low- and high-copy targets
• TRUSTWORTHY – robust performance and 30 day stability at room temperature for extra convenience

Mix Compatibility:

– Bio-Rad: CFX96™, CFX384™, Opticon™, Opticon2™, MiniOpticon, Chromo4™

– Qiagen: Rotor-Gene™ 3000 & 6000

– Eppendorf: Mastercycler® ep realplexMastercycler®

– Roche: LightCycler 480- Bio Molecular Systems: Mic

– Takara: Thermal Cycler Dice (TP800)

Mix Composition:

– SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

– 5x qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction

– SolisGreen® dye: fluorescent dye detected in the FAM channel

Shipping and Storage Conditions:

• Routine storage at -20 °C until expiry date
• The mix can be stored at +4 °C for up to 6 months
• Stability at room temperature (15 – 25 °C) for 1 month
• Freeze-thaw stability: 30 cycles

This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999. SolisFAST is a trademark of Solis Biodyne OÜ. This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999. SolisFAST® is an EU registered trademark of Solis BioDyne OÜ. Other registered trademarks: TaqMan® and LightCycler® (Roche Diagnostics GmbH), Mastercycler® (Eppendorf AG). All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.