SolisFAST® SolisGreen® qPCR Mix (no ROX)
Fast and highly sensitive dye-based qPCR
For in vitro use only
Description:
SolisFAST® SolisGreen® qPCR Mix (no ROX) is a 5x-concentrated ready-to-use solution optimized for fast, highly sensitive and reproducible dye-based qPCR assays. Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start, optimized buffer and the SolisGreen® dye, this qPCR Mix offers robust qPCR, accurate target detection, ice free shipping and reaction set-up.
The mix contains all components necessary, except primers, nuclease-free water, and DNA template.
The mix is compatible with qPCR cyclers that do not need ROX as a passive reference signal for normalization of the data.
Applications:
- DNA/cDNA target detection
- Gene expression analysis
- Copy Number Variation (CNV) analysis
- miRNA profiling and quantification
Benefits:
- FAST - delivers reproducible qPCR results up to 2x faster
- ACCURATE - reliable quantification across technical replicates
- SENSITIVE - consistent results with low- and high-copy targets
- TRUSTWORTHY - robust performance and 30 day stability at room temperature for extra convenience
Mix Compatibility:
- Bio-Rad: CFX96™, CFX384™, Opticon™, Opticon2™, MiniOpticon, Chromo4™
- Qiagen: Rotor-Gene™ 3000 & 6000
- Eppendorf: Mastercycler® ep realplexMastercycler®
- Roche: LightCycler 480- Bio Molecular Systems: Mic
- Takara: Thermal Cycler Dice (TP800)
Mix Composition:
- SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.
- 5x qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
- SolisGreen® dye: fluorescent dye detected in the FAM channel
Shipping and Storage Conditions:
- Routine storage at -20 °C until expiry date
- The mix can be stored at +4 °C for up to 6 months
- Stability at room temperature (15 - 25 °C) for 1 month
- Freeze-thaw stability: 30 cycles
