5x SolisFAST™ Probe qPCR Mix with UNG (ROX)

SolisFAST^® Probe qPCR Mix with UNG (ROX)

For in vitro use only

Description:

SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously. Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice-free shipping and reaction set-up.

Applications:

– Ultra fast DNA/cDNA target detection

– Gene expression analysis

– Copy Number Variation (CNV) analysis

– Targeted SNP genotyping

Benefits:

• FAST – delivers reproducible qPCR results up to 2x faster
• ACCURATE – reliable quantification across technical replicates
• SENSITIVE – consistent results with low- and high-copy targets
• TRUSTWORTHY – robust performance and 30 day stability at room temperature for extra convenience

Mix Compatibility:

– Applied BioSystems: 7500, 7500 Fast, QuantStudio™ 5, 6 Flex, 7 Flex, 12K Flex, ViiA™ 7

Mix Composition:

– SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

– qPCR buffer: Includes 16.5 mM MgCl2 (1X PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR reaction

– ROX dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Shipping and Storage Conditions:

• Routine storage at -20 °C until expiry date
• The mix can be stored at +4 °C for up to 6 months
• Stability at room temperature (15 – 25 °C) for 1 month
• Freeze-thaw stability: 30 cycles

Applied Biosystems is trademarks of Applied Biosystems LLC. QuantStudio and ViiA are trademarks of Life Technologies Corporation.