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5x SolisFAST™ Probe qPCR Mix with UNG (ROX)

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SolisFAST® Probe qPCR Mix with UNG (ROX)

For in vitro use only

Description:

SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously. Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice-free shipping and reaction set-up.

Applications:

- Ultra fast DNA/cDNA target detection

- Gene expression analysis

- Copy Number Variation (CNV) analysis

- Targeted SNP genotyping

Benefits:

  • FAST - delivers reproducible qPCR results up to 2x faster
  • ACCURATE - reliable quantification across technical replicates
  • SENSITIVE - consistent results with low- and high-copy targets
  • TRUSTWORTHY - robust performance and 30 day stability at room temperature for extra convenience

Mix Compatibility:

- Applied BioSystems: 7500, 7500 Fast, QuantStudio™ 5, 6 Flex, 7 Flex, 12K Flex, ViiA™ 7

Mix Composition:

- SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

- qPCR buffer: Includes 16.5 mM MgCl2 (1X PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR reaction

- ROX dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Shipping and Storage Conditions:

  • Routine storage at -20 °C until expiry date
  • The mix can be stored at +4 °C for up to 6 months
  • Stability at room temperature (15 - 25 °C) for 1 month
  • Freeze-thaw stability: 30 cycles
Applied Biosystems is trademarks of Applied Biosystems LLC. QuantStudio and ViiA are trademarks of Life Technologies Corporation.
Variant
Pack/Size
Ordering
Price
Qty
5x SolisFAST™ Probe qPCR Mix with UNG (ROX) - 5 x 1 ml
60 ml (12 x 5-pack (5 x 1 ml)/15 000 rxn) / Vial

SKU: SBD-28-22-00001-5

Manufacturer Part No: 28-22-00001-5

UNSPSC: 41106304

£745.15 ex. VAT
5x SolisFAST™ Probe qPCR Mix with UNG (ROX) - 1 ml
50 ml (50 x 1 ml/12 500 rxn) / Vial

SKU: SBD-28-22-00001

Manufacturer Part No: 28-22-00001

UNSPSC: 41106304

£156.86 ex. VAT
5x SolisFAST™ Probe qPCR Mix with UNG (ROX) - 20 ml
20 ml (5 000 rxn) / Pack

SKU: SBD-28-22-00020

Manufacturer Part No: 28-22-00020

UNSPSC: 41106304

£2,774.98 ex. VAT

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