Solis BioDyne Oö

SolisFAST® Probe qPCR Mix with UNG (no ROX)

  • delivers qPCR results 2x faster (in as little as 30 minutes)
  • reliable quantification in up to 5-plex assays
  • consistent results with low- and high-copy targets
  • robust performance
  • contains dUTP and UNG to prevent carryover contamination
  • tolerant to common PCR inhibitors (read more)
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    Price range: £143.79 through £2,543.73 ex. VAT VAT
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    Description

    SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously.

    Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start and optimized buffer, this qPCR Mix offers accurate target detection and quantification.

    For extra convenience the mix includes dUTP and UNG enzyme that enable UNG treatment to prevent carryover contamination.

    This master mix is developed for TaqMan® probes but is also suitable for other hydrolysis probe types. 

    Applications

    • DNA/LNA hydrolysis probe based assays
    • Detection and quantification of DNA and cDNA targets
    • Profiling gene expression
    • Microbial detection
    • Viral load determination

    Properties

    Concentration: 5x

    Hot-start: yes, 3 minutes initial incubation at 95°C

    Cycling speed: fast or ultra-fast

    Detection type: Probe-based

    Reference dye: Purple

    Multiplex: up to 5 plexes simultaneously

    Compatible real-time instruments: The mix is compatible with qPCR cyclers where Purple dye can be used as a passive reference signal for normalization of fluorescent signal. Check Your cycler!

    Mix Components:

    SolisFAST® DNA PolymeraseIn silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.

    qPCR buffer: Includes 16.5 mM MgCl2 - 1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG and additives that maximize efficiency of PCR 

    Reference dyePurple dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR

    Variant
    Pack/Size
    Ordering
    Price
    Qty
    thumb_8345_105_SolisFASTC2AE_Probe_qPCR_Mix_with_UNG_no_ROX_1ml.jpg
    5x SolisFAST™ Probe qPCR Mix with UNG (no ROX) - 20 ml
    20 ml (5000 rxn) / Pack

    SKU: SBD-28-21-00020

    Manufacturer Part No: 28-21-00020

    UNSPSC: 41106304

    £2,543.73 ex. VAT
    thumb_8345_105_SolisFASTC2AE_Probe_qPCR_Mix_with_UNG_no_ROX_1ml.jpg
    5x SolisFAST™ Probe qPCR Mix with UNG (no ROX) - 1 ml
    1 ml (250 rxn) / Pack

    SKU: SBD-28-21-00001

    Manufacturer Part No: 28-21-00001

    UNSPSC: 41106304

    £143.79 ex. VAT
    thumb_8345_105_SolisFASTC2AE_Probe_qPCR_Mix_with_UNG_no_ROX_1ml.jpg
    5x SolisFAST™ Probe qPCR Mix with UNG (no ROX) - 5 x 1 ml
    5 ml (5-pack: 5 x 1 ml; 1250 rxn) / Pack

    SKU: SBD-28-21-00001-5

    Manufacturer Part No: 28-21-00001-5

    UNSPSC: 41106304

    £683.05 ex. VAT

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