5x SolisFAST™ Probe qPCR Mix (Purple)

SolisFAST^® Probe qPCR Mix (Purple)

Fast and highly sensitive probe-based qPCR

For in vitro use only

Description:

SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously. Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice-free shipping and reaction set-up.

Applications:

– Ultra fast DNA/cDNA target detection

– Gene expression analysis

– Copy Number Variation (CNV) analysis

– Targeted SNP genotyping

Benefits:

• FAST – delivers reproducible qPCR results up to 2x faster
• ACCURATE – reliable quantification across technical replicates
• SENSITIVE – consistent results with low- and high-copy targets
• TRUSTWORTHY – robust performance and 30 day stability at room temperature for extra convenience

Mix Compatibility:

– Applied BioSystems: 7500, 7500 Fast, QuantStudio™ 5, 6 Flex, 7 Flex, 12K Flex, ViiA™ 7

Mix Composition:

– SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

– qPCR buffer: Includes 15 mM MgCl2 (1X PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), UNG, additives that maximize efficiency of PCR reaction

– Purple dye: Internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR. If Cy5 or similar dye is used as one of the fluorophores, Purple passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available.

Shipping and Storage Conditions:

• Routine storage at -20 °C until expiry date
• The mix can be stored at +4 °C for up to 6 months
• Stability at room temperature (15 – 25 °C) for 1 month
• Freeze-thaw stability: 30 cycles

This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999. SolisFAST® is an EU registered trademark of Solis BioDyne OÜ. Other registered trademarks: TaqMan® (Roche Diagnostics GmbH). All trademarks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions.