SolisFAST® Probe qPCR Mix (no ROX)
Fast and highly sensitive probe-based qPCR
For in vitro use only
Description:
SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously. Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice-free shipping and reaction set-up.
Applications:
- Ultra fast DNA/cDNA target detection
- Gene expression analysis
- Copy Number Variation (CNV) analysis
- Targeted SNP genotyping
Benefits:
- FAST - delivers reproducible qPCR results up to 2x faster
- ACCURATE - reliable quantification across technical replicates
- SENSITIVE - consistent results with low- and high-copy targets
- TRUSTWORTHY - robust performance and 30 day stability at room temperature for extra convenience
Mix Compatibility:
- Bio-Rad: CFX96™, CFX384™, Opticon™, Opticon2™, MiniOpticon, Chromo4™, iQ™5, myiQ™
- Qiagen: Rotor-Gene™ 3000, Rotor-Gene™ 6000, Rotor-Gene™ Q
- Eppendorf: Mastercycler® ep realplex
- Roche: LightCycler® 480, LightCycler® 98, LightCycler® Nano- Bio Molecular Systems: Mic
- Takara: Thermal Cycler Dice™ (TP800)
Mix Composition:
- SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.
- qPCR buffer: Includes 15 mM MgCl2 (1X PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR reaction
Shipping and Storage Conditions:
- Routine storage at -20 °C until expiry date
- The mix can be stored at +4 °C for up to 6 months
- Stability at room temperature (15 - 25 °C) for 1 month
- Freeze-thaw stability: 30 cycles
