5x SolisFAST™ Master Mix Ready To Load

SolisFAST^® Master Mix, Ready to Load

Fast singleplex and multiplex endpoint PCR assays

For in vitro use only

Description:

SolisFAST® Master Mix ready to Load is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA). Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up.

Applications:

– Ultra fast endpoint analysis – results in 20 minutes

– Suitable for singleplex and multiplex reactions

– High-yield PCR

– Template generation for sequencing

Benefits:

• FAST – less time from sample to result
• ACCURATE – specific and reliable PCR in singleplex and multiplex assays
• STABLE – 30 day stability at room temperature
• TRUSTWORTHY – avoid carry-over contamination with UNG mixes (available) and get reliable results in sanger sequencing applications

Mix Composition:

– SolisFAST® DNA Polymerase: In silico-designed analogue of Taq DNA Polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild type Taq DNA Polymerase.

– PCR buffer: Includes 17.5 mM MgCl2 (1X PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP) additives that maximize efficiency of PCR reaction

– Green loading dye*: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis.*The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye – TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye – TBE: ~35 bp, TAE: ~45 bp).

Shipping and Storage Conditions:

• Routine storage at -20 °C until expiry date
• The mix can be stored at +4 °C for up to 6 months
• Stability at room temperature (15 – 25 °C) for 1 month
• Freeze-thaw stability: 30 cycles

This product is supplied for research use only. Covered by patent EP2501716, made following the methods of US Patent No 9,321,999. SolisFAST® is an EU registered trademark of Solis BioDyne OÜ.