5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

5x HOT FIREPol^® Probe qPCR Mix Plus (no ROX)

For in vitro use only

Description:

HOT FIREPol^® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol^® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

HOT FIREPol^®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications:

– Detection and quantification of DNA and cDNA targets

– Profiling gene expression

– Microbial detection

– Viral load determination

Mix Compatibility:

– Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon^® 2 & MiniOpticon^®

– Qiagen: Rotor-Gene^® Q, Rotor-Gene^® 6000

– Eppendorf: Mastercycler^®: ep realplex2 & ep realplex4

– Illumina: The Eco™

– Roche: LightCycler^® 480

Mix Composition:

– HOT FIREPol^® DNA Polymerase

– 5x Probe qPCR buffer

– 15 mM MgCl₂ 1x PCR solution – 3 mM

– dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP

– No ROX dye

Shipping and Storage Conditions:

Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® Probe qPCR Mix Plus (no ROX).

Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384, iQ, MyiQ, Chromo4, Opticon 2, MiniOpticon are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc.