Home Products Molecular & Cell Biology PCR and qPCR reagents qPCR Mixes 5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

SKU: - UNSPSC: - Manufacturer Part No: - Pack Size: -
Special Requirements?

Build out your order then request a quote from your cart.

Clear
See Variant info VAT
Ex. Inc.

Product succesfully added to cart. View Cart

If you are looking for a quote, you can request this here.

5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

For in vitro use only

Description:

HOT FIREPol® Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

HOT FIREPol®DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications:

– Detection and quantification of DNA and cDNA targets

– Profiling gene expression

– Microbial detection

– Viral load determination

Mix Compatibility:

Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®

Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000

Eppendorf: Mastercycler®: ep realplex2 & ep realplex4

Illumina: The Eco™

Roche: LightCycler® 480

Mix Composition:

– HOT FIREPol® DNA Polymerase

– 5x Probe qPCR buffer

– 15 mM MgCl2 1x PCR solution – 3 mM

– dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP

– No ROX dye

Shipping and Storage Conditions:

Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (no ROX).

Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384, iQ, MyiQ, Chromo4, Opticon 2, MiniOpticon are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc.

Variant
Pack/Size
Ordering
Price
Qty
7098_Probe_no_rox_web
5× HOT FIREPol® Probe qPCR Mix Plus (no ROX) - 1 ml (250 rxn)
1 ml (250 rxn) / Pack

SKU: SBD-08-15-00001

Manufacturer Part No: 08-15-00001

UNSPSC Code: 41106304

Price: £84.88 ex. VAT
7098_Probe_no_rox_web
5× HOT FIREPol® Probe qPCR Mix Plus (no ROX) - 20 ml (5000 rxn)
20 ml (5000 rxn) / Pack

SKU: SBD-08-15-00020

Manufacturer Part No: 08-15-00020

UNSPSC Code: 41106304

Price: £1,501.45 ex. VAT

Newsletter Sign Up

  • Hidden
  • Hidden
  • Hidden
    DD slash MM slash YYYY
  • Hidden
  • Hidden
  • Hidden
5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

Web Scientific has now joined our sister site Thistle Scientific!

We’re the same great team with the same commitment to quality, but now you can browse a larger range with much greater ease and selling points to say why this is a benefit to you.