5x HOT FIREPol® Probe qPCR Mix Plus (Capillary)

5x HOT FIREPol^® Probe qPCR Mix Plus (Capillary)

For in vitro use only

Description:

HOT FIREPol^® Probe qPCR Mix Plus (Capillary) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol^® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

HOT FIREPol^® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications:

– Detection and quantification of DNA and cDNA targets

– Profiling gene expression

– Microbial detection

– Viral load determination

Mix compatibility:

– Roche: LightCycler^® 1.x, LightCycler^® 2.0

Mix Composition:

– HOT FIREPol^® DNA Polymerase

– 5x Probe qPCR buffer

– 15 mM MgCl₂ 1x PCR solution – 3 mM

– dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP

– BSA

Shipping and Storage Conditions:

Routine storage: -20°C

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® Probe qPCR Mix Plus (Capillary).

LightCycler is a registered trademark of Roche Applied Sciences.