5x HOT FIREPol® Probe qPCR Mix Plus (Capillary)
For in vitro use only
Description:
HOT FIREPol® Probe qPCR Mix Plus (Capillary) is optimized for real-time quantitative PCR assays using sequence-specific fluorogenic DNA-probe chemistries. The Mix contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.
HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Mix compatibility:
- Roche: LightCycler® 1.x, LightCycler® 2.0Mix Composition:
- HOT FIREPol® DNA Polymerase
- 5x Probe qPCR buffer
- 15 mM MgCl2 1x PCR solution – 3 mM
- dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP- BSA
Shipping and Storage Conditions:
Routine storage: -20°C
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (Capillary).
LightCycler is a registered trademark of Roche Applied Sciences.
