5x HOT FIREPol® Multiplex qPCR Mix (Rox)

5x HOT FIREPol^® Multiplex qPCR Mix (ROX)

For in vitro use only

Description:

HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2 and ROX passive reference dye according to system requirements. HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for DNA hydrolysis probes based on the 5′ flap endonuclease activity. HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications:

– Detection and quantification of DNA and cDNA targets

– Profiling gene expression

– Microbial detection

– Viral load determination

Reagents Provided:

– HOT FIREPol® DNA Polymerase

– 5x Multiplex qPCR buffer

– 15 mM MgCl2, 1x PCR solution – 3 mM MgCl2

– dNTPs, including dUTP The mix allows UNG treatment to prevent carryover contamination from previous runs. IMPORTANT: UNG is not included in the 5x HOT FIREPol® Multiplex qPCR Mix and must be purchased separately.

– ROX dye If ROX, Texas Red or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.

Shipping and Storage Conditions:

Routine storage: -20 °C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.