5x HOT FIREPol® GC Master Mix

5x HOT FIREPol® GC Master Mix

For in vitro use only 

Description:

5x HOT FIREPol® GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich templates. Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). 100% DMSO and 25 mM MgCl2 are included in a package in separated vials.

Generated PCR products are compatible with TA cloning procedures

Applications:

- Hot Start GC-rich PCR

- Fragment analysis

- TA cloning

Mix Composition:

- HOT FIREPol^® DNA polymerase

- HOT FIREPol^® GC Buffer

- 7.5 mM MgCl₂ 1x PCR solution – 1.5 mM MgCl₂

- dNTPs

- BSA

In separate vial:

- 100% DMSO

- 25 mM MgCl₂

Advantages of Master Mix:

- decreased set-up time

- reduced risk of contamination

- less pipetting mistakes

- consistent reaction to reaction performance

- contains all reagents required for PCR

Like all our products, the 5x HOT FIREPol^® GC Master Mix is stable at ambient temperature for one month. This makes handling and set-up even more convenient, since no ice has to be used.

Product Performance:

The unique composition of the mix supports robust amplification of broad range of GC-rich targets. A total 14 GC-rich genes were amplified from human genomic DNA, using recommended reaction set-up and cycling protocol for 5x HOT FIREPol^® GC Master Mix.

Mix performs well with GC-rich amplicons up to 3000 bp.

Good results are obtained also with Fragment Analyzis (DAT/SLC6A3).

Shipping and Storage Conditions:

Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of 5x HOT FIREPol^® GC Master Mix.