5x HOT FIREPol® GC Master Mix
For in vitro use only
Description:
5x HOT FIREPol® GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich templates. Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). 100% DMSO and 25 mM MgCl2 are included in a package in separated vials.
Generated PCR products are compatible with TA cloning procedures
Applications:
- Hot Start GC-rich PCR
- Fragment analysis
- TA cloning
Mix Composition:
- HOT FIREPol® DNA polymerase
- HOT FIREPol® GC Buffer
- 7.5 mM MgCl2 1x PCR solution – 1.5 mM MgCl2
- dNTPs
- BSA
In separate vial:
- 100% DMSO
- 25 mM MgCl2
Advantages of Master Mix:
- decreased set-up time
- reduced risk of contamination
- less pipetting mistakes
- consistent reaction to reaction performance
- contains all reagents required for PCR
Like all our products, the 5x HOT FIREPol® GC Master Mix is stable at ambient temperature for one month. This makes handling and set-up even more convenient, since no ice has to be used.
Product Performance:
The unique composition of the mix supports robust amplification of broad range of GC-rich targets. A total 14 GC-rich genes were amplified from human genomic DNA, using recommended reaction set-up and cycling protocol for 5x HOT FIREPol® GC Master Mix.
Mix performs well with GC-rich amplicons up to 3000 bp.
Good results are obtained also with Fragment Analyzis (DAT/SLC6A3).
Shipping and Storage Conditions:
Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of 5x HOT FIREPol® GC Master Mix.
