5x HOT FIREPol® EvaGreen® HRM Mix (no ROX)

5x HOT FIREPol^® EvaGreen^® HRM Mix

For in vitro use only

Description:

5x HOT FIREPol^® EvaGreen^® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen^® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol^® DNA Polymerase, ultrapure dNTPs, MgCl₂ and EvaGreen^® dye. The user simply needs to add water, template and primers.

HOT FIREPol^® DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Applications:

High Resolution Melt (HRM)

Mix Compatibility:

– Bio-Rad: CFX96™ & CFX384™

– Qiagen: Rotor-Gene^® 6000

– Eppendorf: Mastercycler^®: ep realplex2 & ep realplex4

– Illumina: The Eco™

– Roche: LightCycler^® 480

Mix Composition:

– HOT FIREPol^® DNA Polymerase

– 5x EvaGreen^® HRM buffer

– 12.5 mM MgCl₂ 1x PCR solution – 2.5 mM

– dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP

– EvaGreen^® dye

– BSA

EvaGreen^® Dye:

EvaGreen^® is a DNA-binding dye with many features that make it a superior for HRM. Apart from having similar spectra, EvaGreen^® has three important features that set it apart from SYBR^® Green I: EvaGreen^® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen^® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR^® Green or FAM!

Shipping and Storage conditions:

Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® EvaGreen^® HRM Mix.

EvaGreen^® is a registered trademark of BIOTIUM, INC. This product is provided under an agreement between BIOTIUM, INC. and SBD and the manufacture, use, sale or import of this product is subject to one or more of pending patent applications owned by BIOTIUM and ALLELOGIC. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer, where such research does not include testing, analysis or screening services for any third party in return for compensation on a per test basis. The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact BIOTIUM, INC., Business Development, 3423 Investment Blvd, Suite 8, Hayward, CA 94545 Tel: 510-265-1027, Fax: 510-265-1352.”

Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a registered trademark of Corbett Research (Qiagen). CFX96, CFX384 are trademarks of Bio-Rad Laboratories. LightCycler is a registered trademark of Roche Applied Sciences. The Eco is registered trademark of Illumina Inc.