5x HOT FIREPol® Blend Master Mix with 10.0 mM MgCl2

5x HOT FIREPol^® Blend Master Mix

with 10 mM MgCl₂

For in vitro use only

Description:

5x HOT FIREPol^® Blend Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).

HOT FIREPol^® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol^® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol^® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase.

In addition, the mix also allows amplification of longer fragments.

Generated PCR products are compatible with blunt-end and TA cloning procedures (to increase the blunt end cloning efficiency treat the PCR products with T4 DNA polymerase or DNA polymerase I large Klenow fragment prior to cloning).

Applications:

– Hot Start PCR

Mix Composition:

– HOT FIREPol^® DNA polymerase

– Proofreading enzyme

– 5x Blend Master Mix Buffer

– 10 mM MgCl₂ 1x PCR solution – 2 mM MgCl₂

– 1 mM dNTPs of each 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

– BSA

Advantages of Master Mix:

– decreased set-up time

– reduced risk of contamination

– less pipetting mistakes

– consistent reaction to reaction performance

– contains all reagents required for PCR

Like all our products, the 5x HOT FIREPol^® Blend Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

Shipping and Storage Conditions:

Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature or storage for up to 12 months at 2-8°C has no detrimental effects on the quality of 5x HOT FIREPol^® Blend Master Mix.