5x FIREPol® Master Mix with 12.5 mM MgCl2

5x FIREPol® Master Mix

with 12.5 mM MgCl2

For in vitro use only

Description:

5x FIREPol^® Master Mix is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water).

We recommend using 5x FIREPol^® Master Mix in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.

Applications:

– Suited for a wide range of PCR assays

– Primer extension

– TA cloning

Reagent Composition:

– FIREPol^® DNA polymerase

– 5x Reaction Buffer B 0.4 M Tris-HCl, 0.1 M (NH₄)₂SO₄, 0.1% w/v Tween-20

– 12.5 mM MgCl₂ 1x PCR solution – 2.5 mM MgCl₂

– 1 mM dNTPs of each 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP

Advantages of Master Mix:

– decreased set-up time

– reduced risk of contamination

– less pipetting mistakes

– consistent reaction to reaction performance

– contains all reagents required for PCR Like all our products, the 5x FIREPol^® Master Mix is stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

Shipping and Storage Conditions:

Routine storage: -20°C Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8°C has no detrimental effects on the quality of FIREPol^® 5x Master Mix.