A 20X stock solution consists
3M Sodium Chloride
300mM Tris-Sodium Citrate
pH7.0
DNase/RNase non detected.
20X SSC
Saline-sodium citrate buffer
Molecular Biology hybridisation transfer buffer saline-sodium citrate (SSC) buffer is used to control stringency for washing steps in protocols for Southern Blotting, Northern Blotting, in situ hybridisation DNA microarrays. 20X SSC may be used to prevent drying of Agarose and PAGE gels during a vacuum transfer.
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