Available as a 1000ml and 5L pack
Tricine is used as an alternative to Glycine in protein buffer systems.
Supplied as a 10X concentrate, dilute to working strength.
Contents per litre (1x working solution)
Tris 0.1M
Tricine 0.1M
pH 8.3
Used as a running buffer in electrophoresis of proteins in PAGE proteomics studies.
Preferred as the running buffer of choice for low molecular weight separation of proteins and Peptides.
A Tris-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high molecular weights.
Separation pattern is similar to that produced by a conventional buffer systems, but offers improvements with lower mw protein separation.
Available as a 10X solution dilute to 1x for use.
Store at room temperature and dilute just prior to use with 18MOhms DD H2O.
or equivalent.
(See also Tris-Tricine SDS buffer 20-6800-10)
