10X Tris Tricine pH 8.3

10X Tris-Tricine Buffer (2-Aminoethane sulphonic Acid)

Available as a 1000ml and 5L pack

Tricine is used as an alternative to Glycine in protein buffer systems.

Supplied as a 10X concentrate, dilute to working strength.

Contents per litre (1x working solution)

Tris 0.1M

Tricine 0.1M

pH 8.3

Used as a running buffer in electrophoresis of proteins in PAGE proteomics studies.

Preferred as the running buffer of choice for low molecular weight separation of proteins and Peptides.

A Tris-Tricine buffer system this gives improved resolution at lower molecular weights whilst retaining excellent resolution at the high molecular weights.

Separation pattern is similar to that produced by a conventional buffer systems, but offers improvements with lower mw protein separation.

Available as a 10X solution dilute to 1x for use.

Store at room temperature and dilute just prior to use with 18MOhms DD H2O.

or equivalent.

(See also Tris-Tricine SDS buffer 20-6800-10)