Investigate chemotaxis of fast or slow migrating adherent cells and non-adherent cells in 2D or 3D gel matrices
- Real-time chemotaxis measurement in real-time in a 2D or 3D environment
- Stable chemotactic gradients for long-term experiments
- Reproducible results with reliable and user-independent data
Applications
- 2D and 3D chemotaxis assays with fast or slow migrating cells
- Live cell imaging using inverted microscopy
- Chemotaxis of neutrophiles, lymphocytes, and monocytes
- 3D chemotaxis of leukocytes or cancer cells in an ECM-like matrix
- Invasion assays of tumor cells in Matrigel™
- Chemotaxis measurements of adherent and non-adherent cells
Technical Features
- Chamber geometry optimized for cells in 2D and 3D matrices
- Defined linear gradient with long-term stability
- Homogeneous cell distribution at the experimental starting point
- 3 chambers on one slide for parallel assays
- Ideal for collagen gels, hydrogels, Matrigel™, or similar aqueous gels
- Ready to use, i.e., no assembly required
- Lettered and numbered chambers and reservoirs
Specifications Table
Outer dimensions (w x l) | 25.5 x 75.5 mm² |
Chemotaxis chambers on slide | 3 |
Volume per chamber | 120 µl |
Observation area | 2×1 mm² |
Coating area per chamber – When coating full chamber – When coating observation area only |
3.50 cm2 0.27 cm2 |
Distance between chambers | 18.5 mm |
Total height with plugs | 12 mm |
Volume chemoattractant | 30 µl |
Bottom: ibidi Polymer Coverslip |