µ-Plate 96 Well 3D Glass Bottom: #1.5H (170 ±5 μm) D 263 M Schott glass, black plate, sterilized, individually packed
- A high-throughput screening plate with full ANSI/SLAS (SBS) and robotics compatibility
- Brilliant cell visualization without gel meniscus formation
- Cost-effective high-throughput 3D experiments, requiring only 10 µl of gel per well
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If you are looking for a quote, you can request this here.A black µ-Plate with 96 wells and a #1.5H glass coverslip bottom for high-throughput 3D cell culture and tube formation assays
- A high-throughput screening plate with full ANSI/SLAS (SBS) and robotics compatibility
- Brilliant cell visualisation without gel meniscus formation
- Cost-effective high-throughput 3D experiments, requiring only 10 µl of gel per well
Applications
- 3D cell culture with hydrogels (e.g., Collagen, Laminin or Matrigel®)
- High throughput tube formation assays
- Immunofluorescence staining
- Sprouting assays
- Live cell imaging of attached cells inside or on top of a hydrogel matrix
- Super-resolution microscopy (STED, SIM, (F)PALM, (d)STORM) and fluorescence correlation spectroscopy (FCS)
Technical Features
- Meets ANSI/SLAS (SBS) standards
- 96 wells with standard numbering (letters A–H and numbers 1–12)
- Well-in-a-well feature: 4 mm well within a 5 mm well
- Geometry enables pipetting of a homogeneous gel layer*
- Suitable for use with various types of gels* (e.g., Matrigel®, collagen, and agarose)
- Provides homogeneous cell growth
- Compatible with staining and fixation
- Excellent optical properties for microscopy
- Compatible with multi-channel pipettes
- Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
- No autofluorescence
- Sterile with single packaging
- Also available in a slide format: µ-Slide 15 Well 3D or µ-Slide 15 Well Glass Bottom
- Also available with a polymer coverslip bottom: µ-Plate 96 Well 3D
Every Cell in Focus:
The Principle of the µ-Plate 96 Well 3D
The µ-Plate 96 Well 3D has a specialised geometry for the easy, convenient, and reproducible conduction of tube formation assays. It is also ideal for sprouting assays, immunofluorescence staining, and general 3D cell culture.
After the gel has been pipetted into the inner well and given time to solidify, the cells can be seeded on top of it for tube formation analysis. Due to the “well-in-a-well” technology, the amount of gel needed is reduced to only 10 μl per well, which is 10% of the amount used in regular multiwell plates.
Further, no gel meniscus is formed. This ensures the formation of a uniformly thick gel matrix on which all cells are in one optical plane, creating reproducible cell culture conditions. The µ-Plate 96 Well 3D can be used with all common hydrogel matrices, such as Matrigel®, collagen gels, agarose gels, and hyaluronic acid gels.
With its 96 wells, the µ-Plate 96 Well 3D is designed for high throughput assays. For low throughput assays, ibidi provides the μ-Slide 15 Well 3D.
| Length / width | 127.7 / 85.5 mm |
| Height with / without lid | 16.5 / 14.4 mm |
| Well-to-well distance | 9.0 mm |
| Volume inner wells | 10 µl |
| Ø Inner wells | 4 mm |
| Depth inner wells | 0.87 mm |
| Volume upper wells | 70 µl |
| Ø Upper wells | 5 mm |
| Growth area per inner well | 0.125 cm² |
| Coating area per inner well | 0.23 cm² |
| Well clearance | 0.76 mm |
| Bottom: Glass coverslip No. 1.5H, selected quality, 170 µm +/- 5 µm | |
Instructions
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