Applications
- Cultivation and high-resolution microscopy of cells
- Cell manipulation and microinjection using microcapillaries
- Total Interference Reflection Fluorescence (TIRF) and single molecule applications
- Super-Resolution Microscopy (STED, SIM, (F)PALM, (d)STORM) and Fluorescence Correlation Spectroscopy (FCS)
- Immunofluorescence staining
- Widefield and confocal fluorescence microscopy of living and fixed cells
- Live cell imaging over extended time periods
- Transfection assays
- Differential interference contrast (DIC) when using a DIC Lid
Technical Features
- Standard format imaging dish with a 35 mm diameter for cell and tissue culture
- Low walls for a large access area during micromanipulation experiments
- Lid with locking feature for minimal evaporation
- Bottom made from D 263 M Schott glass with a thickness of 170 µm +/- 5 µm
- May require coating to promote cell attachment
- Compatible with staining and fixation solvents
- No autofluorescence
- Fully biocompatible materials
- Also available: µ-Dish 35 mm, low with a #1.5 ibidi Polymer Coverslip bottom with optimized adhesion, for diverse applications
- Also available with high walls: µ-Dish 35 mm, high with an #1.5 ibidi Polymer Coverslip bottom for everyday use and µ-Dish 35 mm, high Glass Bottom for special microscopic applications
Low Walls for Easy Micromanipulation of Cells
The ibidi μ-Dishes with low walls (i.e., μ-Dish 35 mm, low and µ-Dish 35 mm, low Glass Bottom) are perfectly suited for micromanipulation assays, such as intracellular injections, micropipette chemotaxis assays, or tissue treatment and microscopy. Due to the low walls of these µ-Dishes, instrumentation (e.g., electrodes) can be positioned exactly using a micromanipulator. The excellent optical quality makes the ibidi µ-Dishes ideally suited for single-cell handling and high-resolution live cell imaging. For all standard cell culture applications, we also offer the µ-Dish 35 mm, high product family with high walls.
The Coverslip Bottom
The µ-Dish 35 mm, low Glass Bottom comes with a thin #1.5H Glass Coverslip Bottom made from D 263 M Schott borosilicate glass that has the highest optical quality. ibidi developed these glass surfaces specifically for TIRF, super-resolution microscopy, and single molecule microscopy. The dish is also available with an #1.5 ibidi Polymer Coverslip Bottom for optimal cell adhesion and high-resolution microscopy in a variety of assays.
