Ibidi

µ-Slide 8 Well high Bioinert

  • Superior to the standard ultra-low attachment (ULA) surfaces—stable, biologically inert surface for long-term experiments without any cell or biomolecule adhesion
  • Excellent microscopic quality without any autofluorescence—100% surface passivation combined with the highest optical quality of the ibidi Polymer Coverslip
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Bioinert
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A chambered coverslip with 8 individual wells and a non-adherent surface for spheroids, organoids, and suspension cells

Applications:

  • Generation and long-term culture of 3D cell aggregates (e.g., spheroids and organoids) without any scaffold
  • High resolution fluorescence microscopy of organoids, spheroids, embryoid bodies (EBs), and cells in suspension
  • Background-free analysis of cell-cell interactions
  • Prevention of stem cell differentiation due to attachment
  • Culture of suspension cells in a permanently unattached state
  • Self-assembly tumor spheroid formation assays
  • 3D tumor spheroid models

Technical Features:

  • Chambered coverslip with 8 independent wells and a non-removable polymer coverslip-bottom
  • µ-Slide 8 Well high with a non-adherent, passivated Bioinert surface
  • Bioinert surface with long term passivation—superior to standard ULA surfaces:
    • Ready-to-use without prior surface treatment or preparation
    • No adsorption, coating, or binding of proteins, antibodies, enzymes, and other biomolecules
    • Non-cytotoxic, biologically inert, and non-degradable
  • Imaging chamber slide with excellent optical quality for high-end microscopy
  • Individual well walls for minimizing well-to-well crosstalk and contaminations 
  • Compatible with staining and fixation solutions
  • Biocompatible polymer material—no glue, no leaking
  • Also available with the ibidi Polymer Coverslip bottom with a tissue culture-treated surface: µ-Slide 8 Well high ibiTreat for various experiments with adherent cells
  • Also available as an adhesive version without a bottom: sticky-Slide 8 Well high  
  • Also available with a Glass Coverslip bottom:
    µ-Slide 8 Well high Glass Bottom for special microscopic applications
  • Specifications

    Bioinert Surface

    Bioinert surface thickness 200 nm
    Bioinert surface material Polyol-based hydrogel
    Protein coatings Not possible

    µ-Slide 8 Well high

    Outer dimensions (w x l) 25.5 x 75.5 mm²
    Number of wells 8
    Dimensions of wells (w x l x h) 9.4 x 10.7 x 9.3 mm³
    Volume per well 300 µl
    Total height with/without lid 10.8/9.5 mm
    Growth area per well 1.0 cm²
    Coating area per well 2.2 cm²
    Bottom: ibidi Polymer Coverslip with Bioinert surface

    Specifications

    Bioinert Surface

    Bioinert surface thickness 200 nm
    Bioinert surface material Polyol-based hydrogel
    Protein coatings Not possible

    µ-Slide 8 Well high

    Outer dimensions (w x l) 25.5 x 75.5 mm²
    Number of wells 8
    Dimensions of wells (w x l x h) 9.4 x 10.7 x 9.3 mm³
    Volume per well 300 µl
    Total height with/without lid 10.8/9.5 mm
    Growth area per well 1.0 cm²
    Coating area per well 2.2 cm²
    Bottom: ibidi Polymer Coverslip with Bioinert surface

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