20 X Tris buffered saline solution with the detergent Tween 20 (0.5% v/v )
(20x TBST)
For use as a wash buffer during western blotting, ELISA plate washing and other molecular biology and Immunology washing techniques. Supplied sterile Autoclaved and 0.2 micron filtered Product is shipped and stored at room temperature1X Formulation: 137 mM Sodium Chloride, 20 mM Tris, 0.025% Tween-20. Supplied at pH 7.6. (1X TBST)
This is a concentrated solution which is diluted to 1x working strength.
Dilute Tris Buffered Saline with Tween 20 (TBST-20X) to a 1X solution using ddH2O.
This product supplies enough 20X material to make 20 litres of 1X solution.